diff --git a/packages/grafana-data/src/types/featureToggles.gen.ts b/packages/grafana-data/src/types/featureToggles.gen.ts
index 4c1a908d2fd..64596504fce 100644
--- a/packages/grafana-data/src/types/featureToggles.gen.ts
+++ b/packages/grafana-data/src/types/featureToggles.gen.ts
@@ -1066,6 +1066,10 @@ export interface FeatureToggles {
   */
   alertingRuleRecoverDeleted?: boolean;
   /**
+  * Support Application Signals queries in the X-Ray datasource
+  */
+  xrayApplicationSignals?: boolean;
+  /**
   * Enables localization for plugins
   */
   localizationForPlugins?: boolean;
diff --git a/pkg/services/featuremgmt/registry.go b/pkg/services/featuremgmt/registry.go
index 606ed57cea4..727ffe32223 100644
--- a/pkg/services/featuremgmt/registry.go
+++ b/pkg/services/featuremgmt/registry.go
@@ -1834,6 +1834,15 @@ var (
 			HideFromDocs:      true,
 			Expression:        "true", // enabled by default
 		},
+		{
+			Name:              "xrayApplicationSignals",
+			Description:       "Support Application Signals queries in the X-Ray datasource",
+			Stage:             FeatureStageExperimental,
+			Owner:             awsDatasourcesSquad,
+			FrontendOnly:      true,
+			HideFromAdminPage: true,
+			HideFromDocs:      true,
+		},
 		{
 			Name:         "localizationForPlugins",
 			Description:  "Enables localization for plugins",
diff --git a/pkg/services/featuremgmt/toggles_gen.csv b/pkg/services/featuremgmt/toggles_gen.csv
index 816d4fff02b..ef7cbcfdd96 100644
--- a/pkg/services/featuremgmt/toggles_gen.csv
+++ b/pkg/services/featuremgmt/toggles_gen.csv
@@ -241,4 +241,5 @@ unifiedStorageHistoryPruner,experimental,@grafana/search-and-storage,false,false
 localeFormatPreference,experimental,@grafana/grafana-frontend-platform,false,false,false
 unifiedStorageGrpcConnectionPool,experimental,@grafana/search-and-storage,false,false,false
 alertingRuleRecoverDeleted,GA,@grafana/alerting-squad,false,false,true
+xrayApplicationSignals,experimental,@grafana/aws-datasources,false,false,true
 localizationForPlugins,experimental,@grafana/plugins-platform-backend,false,false,false
diff --git a/pkg/services/featuremgmt/toggles_gen.go b/pkg/services/featuremgmt/toggles_gen.go
index 6212edd254a..89e6a99d839 100644
--- a/pkg/services/featuremgmt/toggles_gen.go
+++ b/pkg/services/featuremgmt/toggles_gen.go
@@ -975,6 +975,10 @@ const (
 	// Enables the UI functionality to recover and view deleted alert rules
 	FlagAlertingRuleRecoverDeleted = "alertingRuleRecoverDeleted"
 
+	// FlagXrayApplicationSignals
+	// Support Application Signals queries in the X-Ray datasource
+	FlagXrayApplicationSignals = "xrayApplicationSignals"
+
 	// FlagLocalizationForPlugins
 	// Enables localization for plugins
 	FlagLocalizationForPlugins = "localizationForPlugins"
diff --git a/pkg/services/featuremgmt/toggles_gen.json b/pkg/services/featuremgmt/toggles_gen.json
index ee37ab5b693..b5a04e9938f 100644
--- a/pkg/services/featuremgmt/toggles_gen.json
+++ b/pkg/services/featuremgmt/toggles_gen.json
@@ -4512,6 +4512,21 @@
         "codeowner": "@grafana/hosted-grafana-team"
       }
     },
+    {
+      "metadata": {
+        "name": "xrayApplicationSignals",
+        "resourceVersion": "1743178672620",
+        "creationTimestamp": "2025-03-28T16:17:52Z"
+      },
+      "spec": {
+        "description": "Support Application Signals queries in the X-Ray datasource",
+        "stage": "experimental",
+        "codeowner": "@grafana/aws-datasources",
+        "frontend": true,
+        "hideFromAdminPage": true,
+        "hideFromDocs": true
+      }
+    },
     {
       "metadata": {
         "name": "zanzana",