diff --git a/pkg/api/folder_permission.go b/pkg/api/folder_permission.go
index e4b884a390d..815cf18826e 100644
--- a/pkg/api/folder_permission.go
+++ b/pkg/api/folder_permission.go
@@ -9,6 +9,7 @@ import (
 	"github.com/grafana/grafana/pkg/api/dtos"
 	"github.com/grafana/grafana/pkg/api/response"
 	"github.com/grafana/grafana/pkg/apimachinery/identity"
+	"github.com/grafana/grafana/pkg/apimachinery/utils"
 	"github.com/grafana/grafana/pkg/infra/metrics"
 	contextmodel "github.com/grafana/grafana/pkg/services/contexthandler/model"
 	"github.com/grafana/grafana/pkg/services/dashboards"
@@ -92,6 +93,11 @@ func (hs *HTTPServer) UpdateFolderPermissions(c *contextmodel.ReqContext) respon
 		return apierrors.ToFolderErrorResponse(err)
 	}
 
+	// Block permission changes for folders managed by provisioning
+	if folder.ManagedBy == utils.ManagerKindRepo {
+		return response.Error(http.StatusForbidden, "Cannot update permissions for folders managed by provisioning.", nil)
+	}
+
 	items := make([]*dashboards.DashboardACL, 0, len(apiCmd.Items))
 	for _, item := range apiCmd.Items {
 		items = append(items, &dashboards.DashboardACL{
diff --git a/pkg/api/folder_permission_test.go b/pkg/api/folder_permission_test.go
index d10185a7360..f768936ec50 100644
--- a/pkg/api/folder_permission_test.go
+++ b/pkg/api/folder_permission_test.go
@@ -9,6 +9,7 @@ import (
 	"github.com/stretchr/testify/assert"
 	"github.com/stretchr/testify/require"
 
+	"github.com/grafana/grafana/pkg/apimachinery/utils"
 	"github.com/grafana/grafana/pkg/services/accesscontrol"
 	"github.com/grafana/grafana/pkg/services/accesscontrol/actest"
 	"github.com/grafana/grafana/pkg/services/dashboards"
@@ -149,4 +150,58 @@ func TestHTTPServer_UpdateFolderPermissions(t *testing.T) {
 		assert.Equal(t, http.StatusBadRequest, res.StatusCode)
 		require.NoError(t, res.Body.Close())
 	})
+
+	t.Run("should not be able to update permissions for folders managed by provisioning", func(t *testing.T) {
+		server := SetupAPITestServer(t, func(hs *HTTPServer) {
+			fakeFolderService := foldertest.NewFakeService()
+			fakeFolderService.ExpectedFolder = &folder.Folder{
+				ID:        1,
+				OrgID:     1,
+				UID:       "1",
+				ManagedBy: utils.ManagerKindRepo,
+			}
+			hs.folderService = fakeFolderService
+			hs.folderPermissionsService = &actest.FakePermissionsService{
+				ExpectedPermissions: []accesscontrol.ResourcePermission{},
+			}
+		})
+
+		body := `{"items": [{"role": "Viewer", "permission": 1}]}`
+		res, err := server.SendJSON(webtest.RequestWithSignedInUser(server.NewPostRequest("/api/folders/1/permissions", strings.NewReader(body)), userWithPermissions(1, []accesscontrol.Permission{
+			{Action: dashboards.ActionFoldersPermissionsWrite, Scope: "folders:uid:1"},
+		})))
+
+		require.NoError(t, err)
+		assert.Equal(t, http.StatusForbidden, res.StatusCode)
+
+		var result map[string]interface{}
+		require.NoError(t, json.NewDecoder(res.Body).Decode(&result))
+		assert.Contains(t, result["message"].(string), "Cannot update permissions for folders managed by provisioning")
+		require.NoError(t, res.Body.Close())
+	})
+
+	t.Run("should be able to update permissions for non-provisioned folders", func(t *testing.T) {
+		server := SetupAPITestServer(t, func(hs *HTTPServer) {
+			fakeFolderService := foldertest.NewFakeService()
+			fakeFolderService.ExpectedFolder = &folder.Folder{
+				ID:        1,
+				OrgID:     1,
+				UID:       "1",
+				ManagedBy: utils.ManagerKindUnknown, // Not managed by provisioning
+			}
+			hs.folderService = fakeFolderService
+			hs.folderPermissionsService = &actest.FakePermissionsService{
+				ExpectedPermissions: []accesscontrol.ResourcePermission{},
+			}
+		})
+
+		body := `{"items": [{"role": "Viewer", "permission": 1}]}`
+		res, err := server.SendJSON(webtest.RequestWithSignedInUser(server.NewPostRequest("/api/folders/1/permissions", strings.NewReader(body)), userWithPermissions(1, []accesscontrol.Permission{
+			{Action: dashboards.ActionFoldersPermissionsWrite, Scope: "folders:uid:1"},
+		})))
+
+		require.NoError(t, err)
+		assert.Equal(t, http.StatusOK, res.StatusCode)
+		require.NoError(t, res.Body.Close())
+	})
 }