diff --git a/public/app/features/alerting/unified/hooks/useAlertQueriesStatus.ts b/public/app/features/alerting/unified/hooks/useAlertQueriesStatus.ts
index 4a7b3386a92..af3b744ceaa 100644
--- a/public/app/features/alerting/unified/hooks/useAlertQueriesStatus.ts
+++ b/public/app/features/alerting/unified/hooks/useAlertQueriesStatus.ts
@@ -1,11 +1,18 @@
 import { useMemo } from 'react';
 
 import { getDataSourceSrv } from '@grafana/runtime';
+import { isExpressionReference } from '@grafana/runtime/src/utils/DataSourceWithBackend';
 import { AlertQuery } from 'app/types/unified-alerting-dto';
 
 export function useAlertQueriesStatus(queries: AlertQuery[]) {
   const allDataSourcesAvailable = useMemo(
-    () => queries.every((query) => Boolean(getDataSourceSrv().getInstanceSettings(query.datasourceUid))),
+    () =>
+      queries
+        .filter((query) => !isExpressionReference(query.datasourceUid))
+        .every((query) => {
+          const instanceSettings = getDataSourceSrv().getInstanceSettings(query.datasourceUid);
+          return Boolean(instanceSettings);
+        }),
     [queries]
   );