diff --git a/packages/grafana-prometheus/src/datasource.ts b/packages/grafana-prometheus/src/datasource.ts
index cb4c80f0afd..6c8b1d3ec55 100644
--- a/packages/grafana-prometheus/src/datasource.ts
+++ b/packages/grafana-prometheus/src/datasource.ts
@@ -1,5 +1,6 @@
 // Core Grafana history https://github.com/grafana/grafana/blob/v11.0.0-preview/public/app/plugins/datasource/prometheus/datasource.ts
 import { defaults } from 'lodash';
+import { tz } from 'moment-timezone';
 import { lastValueFrom, Observable, throwError } from 'rxjs';
 import { map, tap } from 'rxjs/operators';
 import semver from 'semver/preload';
@@ -373,13 +374,32 @@ export class PrometheusDatasource
   }
 
   processTargetV2(target: PromQuery, request: DataQueryRequest<PromQuery>) {
+    // The `utcOffsetSec` parameter is required by the backend to correctly align time ranges.
+    // This alignment ensures that relative time ranges (e.g., "Last N hours/days/years") are adjusted
+    // according to the user's selected time zone, rather than defaulting to UTC.
+    //
+    // Example: If the user selects "Last 5 days," each day should begin at 00:00 in the chosen time zone,
+    // rather than at 00:00 UTC, ensuring an accurate breakdown.
+    //
+    // This adjustment does not apply to absolute time ranges, where users explicitly set
+    // the start and end timestamps.
+    //
+    // Handling `utcOffsetSec`:
+    // - When using the browser's time zone, the UTC offset is derived from the request range object.
+    // - When the user selects a custom time zone, the UTC offset must be calculated accordingly.
+    // More details:
+    // - Issue that led to the introduction of utcOffsetSec: https://github.com/grafana/grafana/issues/17278
+    // - Implementation PR: https://github.com/grafana/grafana/pull/17477
+    let utcOffset = request.range.to.utcOffset();
+    if (request.timezone !== 'browser') {
+      utcOffset = tz(request.timezone).utcOffset();
+    }
     const processedTargets: PromQuery[] = [];
     const processedTarget = {
       ...target,
       exemplar: this.shouldRunExemplarQuery(target, request),
       requestId: request.panelId + target.refId,
-      // We need to pass utcOffsetSec to backend to calculate aligned range
-      utcOffsetSec: request.range.to.utcOffset() * 60,
+      utcOffsetSec: utcOffset * 60,
     };
 
     if (config.featureToggles.promQLScope) {