diff --git a/packages/grafana-ui/src/components/GraphNG/utils.test.ts b/packages/grafana-ui/src/components/GraphNG/utils.test.ts
index 23efa7a7726..71b4f7a673e 100644
--- a/packages/grafana-ui/src/components/GraphNG/utils.test.ts
+++ b/packages/grafana-ui/src/components/GraphNG/utils.test.ts
@@ -5,6 +5,7 @@ import {
   DataFrame,
   DefaultTimeZone,
   EventBusSrv,
+  FieldColorModeId,
   FieldConfig,
   FieldMatcherID,
   fieldMatchers,
@@ -38,6 +39,9 @@ function mockDataFrame() {
 
   const f1Config: FieldConfig<GraphFieldConfig> = {
     displayName: 'Metric 1',
+    color: {
+      mode: FieldColorModeId.Fixed,
+    },
     decimals: 2,
     custom: {
       drawStyle: GraphDrawStyle.Line,
@@ -62,6 +66,9 @@ function mockDataFrame() {
 
   const f2Config: FieldConfig<GraphFieldConfig> = {
     displayName: 'Metric 2',
+    color: {
+      mode: FieldColorModeId.Fixed,
+    },
     decimals: 2,
     custom: {
       drawStyle: GraphDrawStyle.Bars,
@@ -87,6 +94,9 @@ function mockDataFrame() {
   const f3Config: FieldConfig<GraphFieldConfig> = {
     displayName: 'Metric 3',
     decimals: 2,
+    color: {
+      mode: FieldColorModeId.Fixed,
+    },
     custom: {
       drawStyle: GraphDrawStyle.Line,
       gradientMode: GraphGradientMode.Opacity,
@@ -110,6 +120,9 @@ function mockDataFrame() {
   const f4Config: FieldConfig<GraphFieldConfig> = {
     displayName: 'Metric 4',
     decimals: 2,
+    color: {
+      mode: FieldColorModeId.Fixed,
+    },
     custom: {
       drawStyle: GraphDrawStyle.Bars,
       gradientMode: GraphGradientMode.Hue,
@@ -133,6 +146,9 @@ function mockDataFrame() {
   const f5Config: FieldConfig<GraphFieldConfig> = {
     displayName: 'Metric 4',
     decimals: 2,
+    color: {
+      mode: FieldColorModeId.Fixed,
+    },
     custom: {
       drawStyle: GraphDrawStyle.Bars,
       gradientMode: GraphGradientMode.Hue,
diff --git a/packages/grafana-ui/src/components/TimeSeries/utils.ts b/packages/grafana-ui/src/components/TimeSeries/utils.ts
index 062378b5124..fe587a7b64f 100644
--- a/packages/grafana-ui/src/components/TimeSeries/utils.ts
+++ b/packages/grafana-ui/src/components/TimeSeries/utils.ts
@@ -14,6 +14,7 @@ import {
   getFieldSeriesColor,
   getFieldDisplayName,
   getDisplayProcessor,
+  FieldColorModeId,
 } from '@grafana/data';
 import {
   AxisPlacement,
@@ -54,7 +55,14 @@ export const preparePlotConfigBuilder: UPlotConfigPrepFn<{
 }) => {
   const builder = new UPlotConfigBuilder(timeZone);
 
-  builder.setPrepData((frames) => preparePlotData2(frames[0], builder.getStackingGroups()));
+  let alignedFrame: DataFrame;
+
+  builder.setPrepData((frames) => {
+    // cache alignedFrame
+    alignedFrame = frames[0];
+
+    return preparePlotData2(frames[0], builder.getStackingGroups());
+  });
 
   // X is the first field in the aligned frame
   const xField = frame.fields[0];
@@ -278,6 +286,12 @@ export const preparePlotConfigBuilder: UPlotConfigPrepFn<{
       }
     }
 
+    let dynamicSeriesColor: ((seriesIdx: number) => string | undefined) | undefined = undefined;
+
+    if (colorMode.id === FieldColorModeId.Thresholds) {
+      dynamicSeriesColor = (seriesIdx) => getFieldSeriesColor(alignedFrame.fields[seriesIdx], theme).color;
+    }
+
     builder.addSeries({
       pathBuilder,
       pointsBuilder,
@@ -287,6 +301,7 @@ export const preparePlotConfigBuilder: UPlotConfigPrepFn<{
       colorMode,
       fillOpacity,
       theme,
+      dynamicSeriesColor,
       drawStyle: customConfig.drawStyle!,
       lineColor: customConfig.lineColor ?? seriesColor,
       lineWidth: customConfig.lineWidth,
diff --git a/packages/grafana-ui/src/components/uPlot/config/UPlotSeriesBuilder.ts b/packages/grafana-ui/src/components/uPlot/config/UPlotSeriesBuilder.ts
index fe0c7f081f3..cddb4918c57 100644
--- a/packages/grafana-ui/src/components/uPlot/config/UPlotSeriesBuilder.ts
+++ b/packages/grafana-ui/src/components/uPlot/config/UPlotSeriesBuilder.ts
@@ -29,6 +29,7 @@ export interface SeriesProps extends LineConfig, BarConfig, FillConfig, PointsCo
   scaleKey: string;
   pxAlign?: boolean;
   gradientMode?: GraphGradientMode;
+  dynamicSeriesColor?: (seriesIdx: number) => string | undefined;
 
   facets?: uPlot.Series.Facet[];
 
@@ -150,7 +151,22 @@ export class UPlotSeriesBuilder extends PlotConfigBuilder<SeriesProps, Series> {
   }
 
   private getLineColor(): Series.Stroke {
-    const { lineColor, gradientMode, colorMode, thresholds, theme, hardMin, hardMax, softMin, softMax } = this.props;
+    const {
+      lineColor,
+      gradientMode,
+      colorMode,
+      thresholds,
+      theme,
+      hardMin,
+      hardMax,
+      softMin,
+      softMax,
+      dynamicSeriesColor,
+    } = this.props;
+
+    if (gradientMode === GraphGradientMode.None && dynamicSeriesColor) {
+      return (plot: uPlot, seriesIdx: number) => dynamicSeriesColor(seriesIdx) ?? lineColor ?? FALLBACK_COLOR;
+    }
 
     if (gradientMode === GraphGradientMode.Scheme && colorMode?.id !== FieldColorModeId.Fixed) {
       return getScaleGradientFn(1, theme, colorMode, thresholds, hardMin, hardMax, softMin, softMax);
@@ -172,6 +188,7 @@ export class UPlotSeriesBuilder extends PlotConfigBuilder<SeriesProps, Series> {
       hardMax,
       softMin,
       softMax,
+      dynamicSeriesColor,
     } = this.props;
 
     if (fillColor) {
@@ -181,6 +198,14 @@ export class UPlotSeriesBuilder extends PlotConfigBuilder<SeriesProps, Series> {
     const mode = gradientMode ?? GraphGradientMode.None;
     const opacityPercent = (fillOpacity ?? 0) / 100;
 
+    if (mode === GraphGradientMode.None && dynamicSeriesColor && opacityPercent > 0) {
+      return (u: uPlot, seriesIdx: number) => {
+        // @ts-ignore
+        let lineColor = u.series[seriesIdx]._stroke; // cache
+        return colorManipulator.alpha(lineColor ?? '', opacityPercent);
+      };
+    }
+
     switch (mode) {
       case GraphGradientMode.Opacity:
         return getOpacityGradientFn((fillColor ?? lineColor)!, opacityPercent);