diff --git a/public/app/plugins/datasource/jaeger/datasource.test.ts b/public/app/plugins/datasource/jaeger/datasource.test.ts
index 38ef7b4f286..f3d3c826e2f 100644
--- a/public/app/plugins/datasource/jaeger/datasource.test.ts
+++ b/public/app/plugins/datasource/jaeger/datasource.test.ts
@@ -101,6 +101,22 @@ describe('JaegerDatasource', () => {
     expect(mock).toHaveBeenCalledWith({ url: `${defaultSettings.url}/api/traces/a%2Fb` });
   });
 
+  it('should trim whitespace from traceid', async () => {
+    const mock = setupFetchMock({ data: [testResponse] });
+    const ds = new JaegerDatasource(defaultSettings);
+    const query = {
+      ...defaultQuery,
+      targets: [
+        {
+          query: 'a/b  ',
+          refId: '1',
+        },
+      ],
+    };
+    await lastValueFrom(ds.query(query));
+    expect(mock).toHaveBeenCalledWith({ url: `${defaultSettings.url}/api/traces/a%2Fb` });
+  });
+
   it('returns empty response if trace id is not specified', async () => {
     const ds = new JaegerDatasource(defaultSettings);
     const response = await lastValueFrom(
diff --git a/public/app/plugins/datasource/jaeger/datasource.ts b/public/app/plugins/datasource/jaeger/datasource.ts
index 9af8bde29f7..a26e755cd98 100644
--- a/public/app/plugins/datasource/jaeger/datasource.ts
+++ b/public/app/plugins/datasource/jaeger/datasource.ts
@@ -79,7 +79,7 @@ export class JaegerDatasource extends DataSourceApi<JaegerQuery, JaegerJsonData>
     let { start, end } = this.getTimeRange(options.range);
 
     if (target.queryType !== 'search' && target.query) {
-      let url = `/api/traces/${encodeURIComponent(this.templateSrv.replace(target.query, options.scopedVars))}`;
+      let url = `/api/traces/${encodeURIComponent(this.templateSrv.replace(target.query.trim(), options.scopedVars))}`;
       if (this.traceIdTimeParams) {
         url += `?start=${start}&end=${end}`;
       }