diff --git a/packages/grafana-data/src/types/featureToggles.gen.ts b/packages/grafana-data/src/types/featureToggles.gen.ts
index 3aaaa4aba42..313683eea5b 100644
--- a/packages/grafana-data/src/types/featureToggles.gen.ts
+++ b/packages/grafana-data/src/types/featureToggles.gen.ts
@@ -71,10 +71,6 @@ export interface FeatureToggles {
   */
   showDashboardValidationWarnings?: boolean;
   /**
-  * Use double quotes to escape keyword in a MySQL query
-  */
-  mysqlAnsiQuotes?: boolean;
-  /**
   * Rule backtesting API for alerting
   */
   alertingBacktesting?: boolean;
diff --git a/pkg/services/featuremgmt/registry.go b/pkg/services/featuremgmt/registry.go
index 69893cb94eb..56f6fa585e0 100644
--- a/pkg/services/featuremgmt/registry.go
+++ b/pkg/services/featuremgmt/registry.go
@@ -104,12 +104,6 @@ var (
 			Stage:       FeatureStageExperimental,
 			Owner:       grafanaDashboardsSquad,
 		},
-		{
-			Name:        "mysqlAnsiQuotes",
-			Description: "Use double quotes to escape keyword in a MySQL query",
-			Stage:       FeatureStageExperimental,
-			Owner:       grafanaSearchAndStorageSquad,
-		},
 		{
 			Name:        "alertingBacktesting",
 			Description: "Rule backtesting API for alerting",
diff --git a/pkg/services/featuremgmt/toggles_gen.csv b/pkg/services/featuremgmt/toggles_gen.csv
index a6f6bb4a480..28b50a0840b 100644
--- a/pkg/services/featuremgmt/toggles_gen.csv
+++ b/pkg/services/featuremgmt/toggles_gen.csv
@@ -11,7 +11,6 @@ logRequestsInstrumentedAsUnknown,experimental,@grafana/grafana-backend-group,fal
 grpcServer,preview,@grafana/search-and-storage,false,false,false
 cloudWatchCrossAccountQuerying,GA,@grafana/aws-datasources,false,false,false
 showDashboardValidationWarnings,experimental,@grafana/dashboards-squad,false,false,false
-mysqlAnsiQuotes,experimental,@grafana/search-and-storage,false,false,false
 alertingBacktesting,experimental,@grafana/alerting-squad,false,false,false
 logsContextDatasourceUi,GA,@grafana/observability-logs,false,false,true
 lokiShardSplitting,experimental,@grafana/observability-logs,false,false,true
diff --git a/pkg/services/featuremgmt/toggles_gen.go b/pkg/services/featuremgmt/toggles_gen.go
index ba6d0d3a415..f31bfc978bf 100644
--- a/pkg/services/featuremgmt/toggles_gen.go
+++ b/pkg/services/featuremgmt/toggles_gen.go
@@ -55,10 +55,6 @@ const (
 	// Show warnings when dashboards do not validate against the schema
 	FlagShowDashboardValidationWarnings = "showDashboardValidationWarnings"
 
-	// FlagMysqlAnsiQuotes
-	// Use double quotes to escape keyword in a MySQL query
-	FlagMysqlAnsiQuotes = "mysqlAnsiQuotes"
-
 	// FlagAlertingBacktesting
 	// Rule backtesting API for alerting
 	FlagAlertingBacktesting = "alertingBacktesting"
diff --git a/pkg/services/featuremgmt/toggles_gen.json b/pkg/services/featuremgmt/toggles_gen.json
index 9fc363536d4..80db44fec7a 100644
--- a/pkg/services/featuremgmt/toggles_gen.json
+++ b/pkg/services/featuremgmt/toggles_gen.json
@@ -2743,7 +2743,8 @@
       "metadata": {
         "name": "mysqlAnsiQuotes",
         "resourceVersion": "1753448760331",
-        "creationTimestamp": "2022-10-12T11:43:35Z"
+        "creationTimestamp": "2022-10-12T11:43:35Z",
+        "deletionTimestamp": "2025-11-18T19:38:17Z"
       },
       "spec": {
         "description": "Use double quotes to escape keyword in a MySQL query",
diff --git a/pkg/services/sqlstore/database_config.go b/pkg/services/sqlstore/database_config.go
index 65cb51d6f04..15679e030a6 100644
--- a/pkg/services/sqlstore/database_config.go
+++ b/pkg/services/sqlstore/database_config.go
@@ -166,11 +166,7 @@ func (dbCfg *DatabaseConfig) buildConnectionString(cfg *setting.Cfg, features fe
 			cnnstr += fmt.Sprintf("&transaction_isolation=%s", val)
 		}
 
-		//nolint:staticcheck // not yet migrated to OpenFeature
-		if features != nil && features.IsEnabledGlobally(featuremgmt.FlagMysqlAnsiQuotes) {
-			cnnstr += "&sql_mode='ANSI_QUOTES'"
-		}
-
+		cnnstr += "&sql_mode='ANSI_QUOTES'"
 		cnnstr += buildExtraConnectionString('&', dbCfg.UrlQueryParams)
 	case migrator.Postgres:
 		addr, err := util.SplitHostPortDefault(dbCfg.Host, "127.0.0.1", "5432")
diff --git a/pkg/services/sqlstore/database_config_test.go b/pkg/services/sqlstore/database_config_test.go
index 4c398a1d183..e180051d6cf 100644
--- a/pkg/services/sqlstore/database_config_test.go
+++ b/pkg/services/sqlstore/database_config_test.go
@@ -100,7 +100,7 @@ var databaseConfigTestCases = []databaseConfigTest{
 		name:       "MySQL with ANSI_QUOTES mode",
 		dbType:     "mysql",
 		dbHost:     "[::1]",
-		features:   featuremgmt.WithFeatures(featuremgmt.FlagMysqlAnsiQuotes),
+		features:   featuremgmt.WithFeatures(),
 		expConnStr: ":@tcp([::1])/test_db?collation=utf8mb4_unicode_ci&allowNativePasswords=true&clientFoundRows=true&parseTime=true&sql_mode='ANSI_QUOTES'",
 	},
 }